In additional chemical stimulation, cell growth and differentiation are also sensitive to physical signals like shear force or strains. We study and develop physical stress bioreactor on microfluidics and mesoscale bioreactor. A highly stretchable conductive polypyrrole film is fabricated for electrical and strain stimulations.
Physical shear stress & chemical shear stress stimulation on human placenta-derived multipotent cells (PDMCs)
Highly stretchable conductive polypyrrole films
Shear and normal stress stimulation microfluidic bioreactor
Wei-Wen Hu, Tun-Chi Chen, Chia-Wen Tsao, and Yu-Che Cheng “The effects of substrate-mediated electrical stimulation on the promotion of osteogenic differentiation and its optimization” The Journal of Biomedical Materials Research Part B: Applied Biomaterials, 2018 1-13
Chia-Wen Tsao*, Yu-Che Cheng, and Jhih-Hao Cheng “Fluid flow shear stress stimulation on a multiplex microfluidic device for rat bone marrow stromal cells differentiation enhancement” Micromachines 2015, 6(12), 1996-2009
Xu-Cheng Guo, Wei-Wen Hu, Say Hwa Tan and Chia-Wen Tsao* “A Stretchable Conductive Polypyrrole Polydimethylsiloxane Device Fabricated by Simple Soft Lithography and Oxygen Plasma Treatment” Biomedical Microdevices. 2018;20(2):30
Chia-Wen Tsao*, Xu-Cheng Guo and Wei-Wen Hu “Highly stretchable conductive polypyrrole film on three dimensional porous polydimethylsiloxane surface fabricated by simple soft lithography process” RSC Advances, 2016, 6, 113344-113351
Chia-Wen Tsao*, Yu-Che Cheng, and Jhih-Hao Cheng “Fluid flow shear stress stimulation on a multiplex microfluidic device for rat bone marrow stromal cells differentiation enhancement” Micromachines 2015, 6(12), 1996-2009
Y.-C. Cheng, C.-W. Tsao*, M.-Z. Chiang, C.-A. Chung, C.-C. Chien, W.-W. Hu, R-C Ruaan and C. Li, “Microfluidic platform for human placenta-derived multipotent stem cells culture and applied for enhanced neuronal differentiation”, Microfluidics and Nanofluidics, (2014) 1-12
Shao-Kuan Chen, Chih-Ang Chung, Yu-Che Cheng, Chi-Jung Huang, Wen-Yih Chen, Ruoh-Chyu Ruaan, Chuan Li, Chia-Wen Tsao, Wei-Wen Hu, and Chih-Cheng Chien “Toll-like Receptor 6 and Connective Tissue Growth Factor are significantly upregulated in mitomycin-C treated urothelial carcinoma cells under hydrostatic pressure stimulation” Genetic Testing and Molecular Biomarkers 18(6):410-6, 2014
Atsushi Hattori, Tohru Yagi and Chia-Wen Tsao “A Study on Encapsulation of Single-Cell into Fixed Droplets Using Triangular Microwells” IEEJ Transactions on Electronics, Information and Systems Vol. 134 (2014) No. 6 P 848-855
Wei-Wen Hu, Yi-Ting Hsu, Yu-Che Cheng, Chuan Li, Ruoh-Chyu Ruaan, Chih-Cheng Chien, Chih-Ang Chung, and Chia-Wen Tsao “Electrical stimulation to promote osteogenesis using conductive polypyrrole films” Materials Science and Engineering C. 37 (2014) 28–36
Shao-Kuan Chen, Chih-Ang Chung, Yu-Che Cheng, Chi-Jung Huang, Ruoh-Chyu Ruaan, Wen-Yih Chen, Chuan Li, Chia-Wen Tsao, Wei-Wen Hu and Chih-Cheng Chien “Hydrostatic pressure enhances mitomycin C induced apoptosis in urothelial carcinoma cells” Urologic Oncology: Seminars and Original Investigations, 32(2014)26.e17–26.e24
Wei-Wen Hu, Wun-Jheng Syu, Wen-Yih Chen, Ruoh-Chyu Ruaan, Yu-Che Cheng, Chih-Cheng Chien, Chuan Li, Chih-Ang Chung and Chia-Wen Tsao “Use of Biotinylated Chitosan for Substrate-Mediated Gene Delivery” Bioconjugate Chem, 2012, 23 (8), pp 1587–1599
Huang, H.I., Cheng, Y.C., Chen W.Y., Tsao, C.W., Huang C.J., Liu, H.T., and Chien, C.C. “Comparative proteomic analysis of placenta-derived multipotent cells (PDMCs) and its differentiated neuron.”The Chinese J. Pain. 2010, 20(1), 01-11.